Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents
نویسندگان
چکیده
Cas13a has been used to target RNA viruses in cell culture, but efficacy not demonstrated animal models. In this study, we messenger (mRNA)-encoded for mitigating influenza virus A and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection mice hamsters, respectively. We designed CRISPR RNAs (crRNAs) specific PB1 highly conserved regions of PB2 virus, against the replicase nucleocapsid genes SARS-CoV-2, selected crRNAs that reduced viral levels most efficiently culture. delivered polymer-formulated mRNA validated guides tract using a nebulizer. mice, degraded lung tissue when after infection, whereas delivery SARS-CoV-2 replication symptoms. Our findings suggest Cas13a-mediated targeting pathogenic can mitigate infections. hamsters inhibits SARS-CoV-2.
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ژورنال
عنوان ژورنال: Nature Biotechnology
سال: 2021
ISSN: ['1546-1696', '1087-0156']
DOI: https://doi.org/10.1038/s41587-021-00822-w